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Cell Applications Inc
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ScienCell
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Lonza
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PELOBIOTECH GmbH
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Bacto Laboratories
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Cambrex
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Difco
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PELOBIOTECH GmbH
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ScienCell
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ScienCell
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Lonza
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Lifeline Cell Technology
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Image Search Results
Journal: Frontiers in Cellular and Infection Microbiology
Article Title: Genomic and phenotypic characterization of a Clostridioides difficile strain of the epidemic ST37 type from China
doi: 10.3389/fcimb.2024.1412408
Figure Lengend Snippet: Sporulation and germination rates of C. difficile strains. (A) Sporulation. *P<0.05; **P<0.01. (B) Germination: Purified C. difficile spores were suspended in buffer supplemented with taurocholic acid and glycine to induce germination. Germination was monitored by plotting the ratio of the OD 600 at a given time to the OD 600 at time zero. Data are from three biological replicates and reported as mean ± SEM. ****p=0.0001(CD630E vs Xy06); ***p=0.0021 (Xy07 vs Xy06); ns: p=0.0714 (R20291 vs Xy06). (C) Spore colony-forming efficiency. Spore suspensions were serially diluted and plated on BHIS agar supplemented with 0.1%(w/v) taurocholic acid (TA). The spore colony-forming efficiency was defined as the percentage of total spores that gave rise to colonies on BHIS agar with 0.1% (w/v) TA, calculated by c.f.u. per ml colony count/c.f.u. per ml direct count by microscopy × 100%. Data are from three biological replicates and reported as mean ± SEM. * p=0.0276 (CD630E vs Xy06); ns: no significance (R20291, Xy07 vs Xy06). ns- not significant.
Article Snippet: A mixture of 70:30
Techniques: Purification, Microscopy